Increasing the activity of immobilized enzymes with nanoparticle conjugation
نویسندگان
چکیده
منابع مشابه
Counting the number of enzymes immobilized onto a nanoparticle-coated electrode
To immobilize enzymes at the surface of a nanoparticle-based electrochemical sensor is a common method to construct biosensors for non-electroactive analytes. Studying the interactions between the enzymes and nanoparticle support is of great importance in optimizing the conditions for biosensor design. This can be achieved by using a combination of analytical methods to carefully characterize t...
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heteropoly acids (hpa) and their salts have advantages as catalysts which make them both economically and environmentally attractive, strong br?nsted acidity, exhibiting fast reversible multi-electron redox transformations under rather mild conditions, very high solubility in polar solvents, fairly high thermal stability in the solid states, and efficient oxidizing ability, so that they are imp...
15 صفحه اولDrinking Water Decontamination with Immobilized Enzymes
Nerve agent-degrading enzymes Organophosphorus Acid Anhydrolase (OPAA) and Organophosphorus Hydrolase (OPH) covalently-coupled to solid supports were examined as drinking water system nerve agent decontaminants. Enzymes were bound to azlactone polyacrylamide, glyoxal agarose and glyoxal-aminopropyl controlled-pore glass and tested for stability in unbuffered tap water. Kinetic analyses showed t...
متن کاملAnalytical applications of immobilized enzymes.
Several clinical laboratory methods using enzymes as reagents now may utilize immobilized enzymes. These are enzymes attached to solid surfaces by adsorption, covalent binding, cross linking or similar means. Immobilized enzymes are widely used presently in convenient tests for urine glucose and galactose or blood glucose and urea, and in serum glucose or urea determinations by automated method...
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ژورنال
عنوان ژورنال: Current Opinion in Biotechnology
سال: 2015
ISSN: 0958-1669
DOI: 10.1016/j.copbio.2015.04.005